Fig. 1

The Molecular Inversion Probe: Target Generation and Hybridization Procedures a Annealing: Probe and gDNA hybridization; b Gap filling with A/T or G/C nucleotides; c Exonuclease selection for gap filled probes; d Cleavage at site 1 for probe opening and inversion; e Probe amplification and biotinylation; f Cleavage at site 2 to release the tag sequence; f Array hybridization followed by staining with phycoerythrin through the biotin-streptavidin interaction; g Array scanning. Yellow and white colors indicate presence and absence of the phycoerythrin fluorescence signal, respectively. (See text for details)