Table 2 Primary serum, clones, source, working titers, and antigen retrieval
From: Profile of apoptotic proteins in oral squamous cell carcinoma: A cluster analysis of 171 cases
Primary serum | Clone | Source | Working titer | Antigen retrieval |
|---|---|---|---|---|
Bcl-2 | 124 | Dako | 1:200 | Citrate pH 6.0 |
Bcl-x | polyclonal | Dako | 1:500 | Citrate pH 6.0 |
Bcl-xL | 7D9 | Chemicon | 1:2000 | Citrate pH 6.0 |
Bcl-2 related protein A1 | EP517Y | Epitomics | 1:150 | Citrate pH 6.0 |
BAG-1 | 3.10G3E2 | Neomarkers | 1:100 | EDTA/Tris pH 9.0 |
Bak | polyclonal | Dako | 1:300 | Citrate pH 6.0 |
Bax | polyclonal | Dako | 1:400 | Citrate pH 6.0 |
Bim/Bod | polyclonal | Neomarkers | 1:150 | EDTA/Tris pH 9.0 |
Bim Long | 5E5 | Chemicon | 1:100 | Citrate pH 6.0 |
Bad | Y208 | Epitomics | 1:1500 | EDTA/Tris pH 9.0 |
Bid | BH3 | Epitomics | 1:200 | Citrate pH 6.0 |
PUMA | EP512Y | Epitomics | 1:250 | Citrate pH 6.0 |
APAF1 | polyclonal | Novocastra | 1:30 | Citrate pH 6.0 |
Caspase 2 | Y154 | Epitomics | 1:100 | EDTA/Tris pH 9.0 |
Cleaved Caspase 3 | polyclonal | Cell Signaling | 1:600 | Citrate pH 6.0 |
Caspase 6(c-term) | E180 | Epitomics | 1:150 | EDTA/Tris pH 9.0 |
Caspase 7 | 7CSP01 | Chemicon | 1:150 | EDTA/Tris pH 9.0 |
Caspase 8 | 11B6 | Novocastra | 1:250 | Citrate pH 6.0 |
Caspase 9p10 | H83 | Santa Cruz | 1:500 | Citrate pH 6.0 |
Caspase 10 pro | E35 | Epitomics | 1:600 | Citrate pH 6.0 |
Smac/Diablo | Y12 | Epitomics | 1:100 | Citrate pH 6.0 |
Cytochrome c | CTC05 | Chemicon | 1:3000 | Citrate pH 6.0 |
Survivin | polyclonal | Neomarkers | 1:400 | EDTA pH 8.0 |