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Table 2 Primary serum, clones, source, working titers, and antigen retrieval

From: Profile of apoptotic proteins in oral squamous cell carcinoma: A cluster analysis of 171 cases

Primary serum Clone Source Working titer Antigen retrieval
Bcl-2 124 Dako 1:200 Citrate pH 6.0
Bcl-x polyclonal Dako 1:500 Citrate pH 6.0
Bcl-xL 7D9 Chemicon 1:2000 Citrate pH 6.0
Bcl-2 related protein A1 EP517Y Epitomics 1:150 Citrate pH 6.0
BAG-1 3.10G3E2 Neomarkers 1:100 EDTA/Tris pH 9.0
Bak polyclonal Dako 1:300 Citrate pH 6.0
Bax polyclonal Dako 1:400 Citrate pH 6.0
Bim/Bod polyclonal Neomarkers 1:150 EDTA/Tris pH 9.0
Bim Long 5E5 Chemicon 1:100 Citrate pH 6.0
Bad Y208 Epitomics 1:1500 EDTA/Tris pH 9.0
Bid BH3 Epitomics 1:200 Citrate pH 6.0
PUMA EP512Y Epitomics 1:250 Citrate pH 6.0
APAF1 polyclonal Novocastra 1:30 Citrate pH 6.0
Caspase 2 Y154 Epitomics 1:100 EDTA/Tris pH 9.0
Cleaved Caspase 3 polyclonal Cell Signaling 1:600 Citrate pH 6.0
Caspase 6(c-term) E180 Epitomics 1:150 EDTA/Tris pH 9.0
Caspase 7 7CSP01 Chemicon 1:150 EDTA/Tris pH 9.0
Caspase 8 11B6 Novocastra 1:250 Citrate pH 6.0
Caspase 9p10 H83 Santa Cruz 1:500 Citrate pH 6.0
Caspase 10 pro E35 Epitomics 1:600 Citrate pH 6.0
Smac/Diablo Y12 Epitomics 1:100 Citrate pH 6.0
Cytochrome c CTC05 Chemicon 1:3000 Citrate pH 6.0
Survivin polyclonal Neomarkers 1:400 EDTA pH 8.0
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