Table 2 Primer sequences and concentrations
From: SIVA, a target of p53, is downregulated in myelodysplastic syndromes
Gene | Sequence | Concentration |
|---|---|---|
SIVA1 | FW: 5′- TCTTCGAGAAGACCAAGCG −3’ | 300 nM |
RV: 5′- TGCCCAAGGCTCCTGATC −3’ | ||
SIVA2 | FW: 5′- CAGGAGGTCTTCGACCCA −3’ | 600 nM |
RV: 5′- AGTCCACGAGGCCACACA −3’ | ||
TP53 | FW: 5′- GGCGCACAGAGGAAGAGAAT −3’ | 150 nM |
RV: 5′- GGAGAGGAGCTGGTGTTGTTG −3’ | ||
MDM2 | FW: 5′- TTCGAGCCTAGCAATGATCTAGAA −3’ | 150 nM |
RV: 5′- AAACCCACACAACAAATTGCAA −3’ | ||
HPRT1 | FW: 5′- GAACGTCTTGCTCGAGATGTG −3’ | 150 nM |
RV: 5′- TCCAGCAGGTCAGCAAAGAAT-3’ |