Fig. 4

Histopathology of melanoma tumor bearing C57BL/6 mice were subcutaneously transplanted with B16F10 cell line. A portion of excised tumor tissue from melanoma tumor bearing mice were fixed in 10% formalin, cut into 5-μm thickness, stained using H&E (hematoxylin and eosin) and then examined for histopathological changes. a The group which received cyclophosphamide (Positive control) showed equal quantity of both necrotic cells and tumor live cells but when the (b) Tumor bearing animals treated with B. variegata extract shows reduction in the live tumor cells and tumor mass; B. variegata (leaf, stem bark and flower) extract was given along with/without cyclophosphamide showed some histological changes such as (c) full necrosis with focal area of live cells, d Melanoma tumor suggests that total live tumor cells and (e) focal area of live cells with necrosis are present in the tumor control group (TC); (f) full necrosis and vivality with focal area of necrosis. Whereas: LTC – live Tumor cells, NC – Necrotic Cells