Fig. 1
From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
Effect of the DNA template ratio on the genotyping errors of HER2 Ile655Val AS-PCR. AS-PCR was done with two replicates. a the amount of DNA template with GG genotype was kept constant while the amount of DNA template with AA genotype vary, b vice versa. Red arrows indicated preferential amplification, blue arrows indicated LDO, yellow arrows indicated ADO. Line-M is 100 bp DNA ladder. DNA template for AS-PCR used two types of genomic DNA that each contain either A variant of HER2 allele or G variant of HER2 allele. Annealing temperature used in this AS-PCR was at 54.3 °C