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Fig. 2 | Applied Cancer Research

Fig. 2

From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors

Fig. 2

Effect of betaine addition on HER2 Ile655Val AS-PCR performance in eliminating the preferential amplification in mixed DNA template model. This experiment used DNA template consisting of genomic DNA with A variant of HER2 I655V and G variant of HER2 I655V to mimic AG genotype of HER2 Ile655Val in nature. Allele-specific PCR was done with five replicates. Temperature annealing used in this AS-PCR was at 54.3 °C

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