Fig. 3
From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
a effect of DNA template reducing in mixed DNA template model on preferential amplification of HER2 Ile655Val AS-PCR, b effect of the HER2 Ile655Val AS-PCR modification on eliminating the preferential amplification in mixed DNA template model (1 ng AA genotype: 0.25 ng GG genotype). Allele-specific PCR was done with five replicates. Temperature annealing used in this AS-PCR was at 54.3 °C