Fig. 5
From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
Effect of the modified HER2 Ile655Val AS-PCR on preferential PCR amplification. a; Screening of AG genotype samples of genotyping errors-showing patients with breast cancer. b; AS-PCR product of sample No.3 after modifying the denaturation temperature and annealing time. c; AS-PCR product of sample No. 4 after adding betaine in PCR reaction. d; AS-PCR product of sample No. 4 after adding 0.5 ng of DNA template in PCR reaction. AS-PCR was done with three replicates of reactions using annealing temperature of 54.3 °C