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Table 1 List of primers used in this study [13]

From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors

Primer nucleotide sequence Tm primer (°C) Amplicon Size (bp) Primer conc.(μM) in AS-PCR
Her2-AA-Forward 5′-CCAGCCCTCTGACGTCCAGCA-3’ 63.9 142 0.6
Her2-GG-Forward 5’GCGGGCAGGGCGGCGGGGGCGGGGCCCCAGCCCTCTGACGTCCACCG-3’ 83 168 0.06
Her2-Reverse 5′-TCCGTTTCCTGCAGCAGTCTCC-3’ 61.5   0.15