Table 1 List of primers used in this study [13]
From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
Primer | nucleotide sequence | Tm primer (°C) | Amplicon Size (bp) | Primer conc.(μM) in AS-PCR |
|---|---|---|---|---|
Her2-AA-Forward | 5′-CCAGCCCTCTGACGTCCAGCA-3’ | 63.9 | 142 | 0.6 |
Her2-GG-Forward | 5’GCGGGCAGGGCGGCGGGGGCGGGGCCCCAGCCCTCTGACGTCCACCG-3’ | 83 | 168 | 0.06 |
Her2-Reverse | 5′-TCCGTTTCCTGCAGCAGTCTCC-3’ | 61.5 |  | 0.15 |