Table 2 Effects of PCR-related factors on genotyping errors of HER2I655V AS-PCR for homozygous types
From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors
Type of error | DNA template concentration (ng)a | Annealing temperature (°C)b | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
0.4 | 0.2 | 0.1 | 51.1 | 57.7 | |||||||
Experiments | 1th | 2nd | 1th | 2nd | 1th | 2nd | 1th | 2nd | 1th | 2nd | |
AA genotype | |||||||||||
LDO | No PRC Product | 2 | 0 | 0 | 2 | 1 | 3 | 1 | 3 | 3 | 2 |
Correct genotype | 3 | 5 | 5 | 3 | 4 | 2 | 4 | 2 | 2 | 3 | |
Total | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |
Mean of genotyping error rate (%) | 20% | 20% | 40% | 30% | 50% | ||||||
GG genotype | |||||||||||
LDO | No PRC product | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 1 | 0 | 2 |
Correct genotype | 4 | 4 | 4 | 3 | 3 | 3 | 3 | 4 | 5 | 3 | |
total | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |
Mean of genotyping error rate (%) | 20% | 30% | 40% | 30% | 20% | ||||||