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Table 2 Effects of PCR-related factors on genotyping errors of HER2I655V AS-PCR for homozygous types

From: Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors

Type of error   DNA template concentration (ng)a Annealing temperature (°C)b
   0.4 0.2 0.1 51.1 57.7
Experiments   1th 2nd 1th 2nd 1th 2nd 1th 2nd 1th 2nd
AA genotype
 LDO No PRC Product 2 0 0 2 1 3 1 3 3 2
 Correct genotype 3 5 5 3 4 2 4 2 2 3
 Total 5 5 5 5 5 5 5 5 5 5
 Mean of genotyping error rate (%) 20% 20% 40% 30% 50%
GG genotype
 LDO No PRC product 1 1 1 2 2 2 2 1 0 2
 Correct genotype 4 4 4 3 3 3 3 4 5 3
 total 5 5 5 5 5 5 5 5 5 5
 Mean of genotyping error rate (%) 20% 30% 40% 30% 20%
  1. aAllele-specific PCR was done using annealing temperatue of 54.3 °C
  2. bAllele-specific PCR was done using DNA template of 0.4 ng for GG genotype or AA genotype