Table 2 Characteristics of primers used to study KLK7 expression
From: Down regulation of KLK7 expression in breast tissues and identification of a novel spliced KLK7 mRNA
Sr. no. | Gene | Primer | Position of hybridization (Exon: position) | Sequence of primer | Tm (°C) | Length (nt) | Nature of primer |
|---|---|---|---|---|---|---|---|
1 | KLK7 | F1 | E1: 11–28 | AGTCGGAACTTGGATCAC | 52.5 | 18 | sense |
2 | R1 | E6: 6356–6373 | AACTCAGTGTGGCGTTAG | 53.0 | 18 | antisense | |
3 | F2 | E2:1532–1550 | CTGCTATCCTTAGCCTTGG | 53.5 | 19 | sense | |
4 | R2 | E6:6313–6330 | TCCACTTGGTGAACTTGC | 53.6 | 18 | antisense | |
5 | β-actin | F1 | E4: 2253–2271 | TGCGTGACATTAAGGAGAA | 51.5 | 19 | sense |
6 | R1 | E4: 2407–2424 | AAGGAAGGCTGGAAGAGT | 53.9 | 18 | antisense |