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Table 2 Characteristics of primers used to study KLK7 expression

From: Down regulation of KLK7 expression in breast tissues and identification of a novel spliced KLK7 mRNA

Sr. no. Gene Primer Position of hybridization
(Exon: position)
Sequence of primer Tm (°C) Length (nt) Nature of primer
1 KLK7 F1 E1: 11–28 AGTCGGAACTTGGATCAC 52.5 18 sense
2 R1 E6: 6356–6373 AACTCAGTGTGGCGTTAG 53.0 18 antisense
3 F2 E2:1532–1550 CTGCTATCCTTAGCCTTGG 53.5 19 sense
4 R2 E6:6313–6330 TCCACTTGGTGAACTTGC 53.6 18 antisense
5 β-actin F1 E4: 2253–2271 TGCGTGACATTAAGGAGAA 51.5 19 sense
6 R1 E4: 2407–2424 AAGGAAGGCTGGAAGAGT 53.9 18 antisense