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Table 2 Primary antibodies, clones, dilutions, sources, and positive controls used in immunohistochemistry

From: Prognostic implications of the phosphatidylinositol 3-kinase/Akt signaling pathway in oral squamous cell carcinoma: overexpression of p-mTOR indicates an adverse prognosis

Antibodies Clones Dilution Sources Positive Controls
mTOR (phospho) 49F9 1:100 Cell-Signaling Normal breast
PI3K policlonal Ready to use Abcam Placenta
MAPK p44/42 (phospho) D13.14.45 1:50 Cell-Signaling Normal breast
PTEN 6H2.1 1:500 Cascade Normal thyroid
AKT (phospho) 587F11 1:25 Cell-Signaling Colon Tumor