Table 2 Primary antibodies, clones, dilutions, sources, and positive controls used in immunohistochemistry
Antibodies | Clones | Dilution | Sources | Positive Controls |
|---|---|---|---|---|
mTOR (phospho) | 49F9 | 1:100 | Cell-Signaling | Normal breast |
PI3K | policlonal | Ready to use | Abcam | Placenta |
MAPK p44/42 (phospho) | D13.14.45 | 1:50 | Cell-Signaling | Normal breast |
PTEN | 6H2.1 | 1:500 | Cascade | Normal thyroid |
AKT (phospho) | 587F11 | 1:25 | Cell-Signaling | Colon Tumor |