Fig. 4
From: Splicing isoform-specific functional genomic in cancer cells
Workflow to validate isoform-specific tool. STEP 1: The splicing isoform detection pipeline is used to select cancer cell lines with adequate splicing isoform ratio and expression. STEP 2: Splicing Modulator (SM) and Isoform-Specific Inhibitor (ISI) are designed and transfected in the desired cancer cell lines. STEP 3: The splicing isoform detection pipeline is used to quantify the splicing shift and validate isoform-specific tools. If needed, SM and ISI are re-designed