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Table 3 Phenotypic analysis of human cancer cell lines transfected with ISIs targeting cancer-related genes

From: Splicing isoform-specific functional genomic in cancer cells

Gene ASE type Isoform targeted Phenotype Ref. Gene ASE type Isoform targeted Phenotype Ref.
NEK2 Intron retention Long
(NEK2B)
Induce mitotic delay in HeLa cells [96] CCND1 Alt5’ Long (isoform b) Suppress proliferation and invasiveness of SBTB1A and T24 cells [97]
H-Ras cassette Long
(p19)
Induce cell proliferation in HeLa cells [98] CPEB2B cassette Long
(Exon 4)
Sensitized the AnR cell lines to detachment-induced cell death. [99]
BIRC7 Alt3’ short
(isoform β)
Decrease cell proliferation in HeLa cells [100] PKM2 Mutually exclusive M2 Induce apoptosis of multiple cancer cell lines [101]
CCND1 Intron
Retention
Long
(isoform b)
Decrease cell invasion in T24 cells [102] RREB1 cassette Long
(exon 9)
Decrease UMUC-3 cell growth [103]
EIF4H cassette Long
(isoforme 1)
Decrease cell proliferation in LOVO cells [104] WT1 Alt5’ Long
(Exon4a)
Attenuate apoptosis [105]
RPAP3 cassette Short
(isoform 2)
Attenuate doxorubicin-induced cell death in T47D cells [106] RPS6KB1 Multiple exon cassette short Inhibit growth and induce apoptosis [107]
BRD8 cassette Long
(p120β)
Decrease thyroid receptor activation in PC-3 cells [108] GHRL cassette Ln1-ghrelin Reduce cell viability [109]
KLF6 Alt5’ and cryptic 3’ss short
(isoform SV1)
Induce apoptosis in A549 cells [110] CD44 cassette Exonv6 Reduce MBM cell migration [111]
PKC Alt5’ Long
(PKCδVIII)
Induce apoptosis in NT2 cells [68] TP53 cassette 9by Increase MCF-7 cell growth [112]
CXCR3 Alt3’ Long
(isoform B)
Increase proliferation in MDA-MB-435 cells [113] RAP1GDS1 cassette smgGDS-558 Decrease proliferation in multiple breast cancer cell lines [67]
CD99 cassette short Decrease invasion of MDA-MB-435 cells [114] UGT1A cassette i2s Sensitize HT-115 cells to drug-induced cell death [115]
MDM2 Complex Long
(DMX211)
Decrease proliferation of ARO cells [116] MYLK cassette Long
(Isoform 1)
Increase trans-epithelial resistance [117]
  1. The ASE type, isoform targeted and resulting phenotype is described. Ref. Reference