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Table 3 Phenotypic analysis of human cancer cell lines transfected with ISIs targeting cancer-related genes

From: Splicing isoform-specific functional genomic in cancer cells

Gene ASE type Isoform targeted Phenotype Ref. Gene ASE type Isoform targeted Phenotype Ref.
NEK2 Intron retention Long
Induce mitotic delay in HeLa cells [96] CCND1 Alt5’ Long (isoform b) Suppress proliferation and invasiveness of SBTB1A and T24 cells [97]
H-Ras cassette Long
Induce cell proliferation in HeLa cells [98] CPEB2B cassette Long
(Exon 4)
Sensitized the AnR cell lines to detachment-induced cell death. [99]
BIRC7 Alt3’ short
(isoform β)
Decrease cell proliferation in HeLa cells [100] PKM2 Mutually exclusive M2 Induce apoptosis of multiple cancer cell lines [101]
CCND1 Intron
(isoform b)
Decrease cell invasion in T24 cells [102] RREB1 cassette Long
(exon 9)
Decrease UMUC-3 cell growth [103]
EIF4H cassette Long
(isoforme 1)
Decrease cell proliferation in LOVO cells [104] WT1 Alt5’ Long
Attenuate apoptosis [105]
RPAP3 cassette Short
(isoform 2)
Attenuate doxorubicin-induced cell death in T47D cells [106] RPS6KB1 Multiple exon cassette short Inhibit growth and induce apoptosis [107]
BRD8 cassette Long
Decrease thyroid receptor activation in PC-3 cells [108] GHRL cassette Ln1-ghrelin Reduce cell viability [109]
KLF6 Alt5’ and cryptic 3’ss short
(isoform SV1)
Induce apoptosis in A549 cells [110] CD44 cassette Exonv6 Reduce MBM cell migration [111]
PKC Alt5’ Long
Induce apoptosis in NT2 cells [68] TP53 cassette 9by Increase MCF-7 cell growth [112]
CXCR3 Alt3’ Long
(isoform B)
Increase proliferation in MDA-MB-435 cells [113] RAP1GDS1 cassette smgGDS-558 Decrease proliferation in multiple breast cancer cell lines [67]
CD99 cassette short Decrease invasion of MDA-MB-435 cells [114] UGT1A cassette i2s Sensitize HT-115 cells to drug-induced cell death [115]
MDM2 Complex Long
Decrease proliferation of ARO cells [116] MYLK cassette Long
(Isoform 1)
Increase trans-epithelial resistance [117]
  1. The ASE type, isoform targeted and resulting phenotype is described. Ref. Reference