Splicing isoform-specific functional genomic in cancer cells

Brosseau, Jean-Philippe

doi:10.1186/s41241-018-0068-6

Applied Cancer Research

Table 3 Phenotypic analysis of human cancer cell lines transfected with ISIs targeting cancer-related genes

From: Splicing isoform-specific functional genomic in cancer cells

Gene	ASE type	Isoform targeted	Phenotype	Ref.	Gene	ASE type	Isoform targeted	Phenotype	Ref.
NEK2	Intron retention	Long (NEK2B)	Induce mitotic delay in HeLa cells	[96]	CCND1	Alt5’	Long (isoform b)	Suppress proliferation and invasiveness of SBTB1A and T24 cells	[97]
H-Ras	cassette	Long (p19)	Induce cell proliferation in HeLa cells	[98]	CPEB2B	cassette	Long (Exon 4)	Sensitized the AnR cell lines to detachment-induced cell death.	[99]
BIRC7	Alt3’	short (isoform β)	Decrease cell proliferation in HeLa cells	[100]	PKM2	Mutually exclusive	M2	Induce apoptosis of multiple cancer cell lines	[101]
CCND1	Intron Retention	Long (isoform b)	Decrease cell invasion in T24 cells	[102]	RREB1	cassette	Long (exon 9)	Decrease UMUC-3 cell growth	[103]
EIF4H	cassette	Long (isoforme 1)	Decrease cell proliferation in LOVO cells	[104]	WT1	Alt5’	Long (Exon4a)	Attenuate apoptosis	[105]
RPAP3	cassette	Short (isoform 2)	Attenuate doxorubicin-induced cell death in T47D cells	[106]	RPS6KB1	Multiple exon cassette	short	Inhibit growth and induce apoptosis	[107]
BRD8	cassette	Long (p120β)	Decrease thyroid receptor activation in PC-3 cells	[108]	GHRL	cassette	Ln1-ghrelin	Reduce cell viability	[109]
KLF6	Alt5’ and cryptic 3’ss	short (isoform SV1)	Induce apoptosis in A549 cells	[110]	CD44	cassette	Exonv6	Reduce MBM cell migration	[111]
PKC	Alt5’	Long (PKCδVIII)	Induce apoptosis in NT2 cells	[68]	TP53	cassette	9by	Increase MCF-7 cell growth	[112]
CXCR3	Alt3’	Long (isoform B)	Increase proliferation in MDA-MB-435 cells	[113]	RAP1GDS1	cassette	smgGDS-558	Decrease proliferation in multiple breast cancer cell lines	[67]
CD99	cassette	short	Decrease invasion of MDA-MB-435 cells	[114]	UGT1A	cassette	i2s	Sensitize HT-115 cells to drug-induced cell death	[115]
MDM2	Complex	Long (DMX211)	Decrease proliferation of ARO cells	[116]	MYLK	cassette	Long (Isoform 1)	Increase trans-epithelial resistance	[117]

The ASE type, isoform targeted and resulting phenotype is described. Ref. Reference

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