Fig. 1

Digital scans of whole sections of prostate cancer with known PTEN gene status. Each tumor had previously had the status of PTEN status determined using FISH and IHC analysis of TMA cores (small solid turquoise circles). In each panel, areas containing tumor are outlined with a solid green line. Magenta, yellow and blue pseudocoloring refer to high, moderate, and weak expression levels of the PTEN protein. a Gleason 3 + 4 tumor with a large area exhibiting uniformly weak PTEN protein levels (black arrow) and homozygous PTEN deletions were found on both cores 1 and 2 by FISH. b Gleason 3 + 4 tumor in which core 1 had homozygous PTEN deletions by FISH. This core was taken from an area with weak PTEN protein levels (black arrow) that was surrounded by a large area with moderate expression (yellow). Core 2 of this tumor from the contralateral lobe also has moderate PTEN protein levels (black arrow) and both PTEN genes were intact by FISH. Two regions (black arrows) either side of core 2 have weak levels of PTEN protein. c Gleason 3 + 4 tumor in which core 1 had a homozygous PTEN deletions with weak PTEN protein (black arrow) present in about 50% of the encircled area containing tumor. A small patch of tumor to the left also has moderate levels of PTEN protein. d Gleason 4 + 3 tumor in which both PTEN genes were intact by FISH in core 1 and protein levels were high. Core 2 was had a hemizygous PTEN deletion with weak PTEN protein levels. e Gleason 4 + 3 tumor with hemizygous PTEN gene deletions in both cores 1 and 2 and protein levels were moderate-high in the entire area of tumor. f Gleason 4 + 3 tumor with hemizygous PTEN gene deletions in core 1 and protein levels were moderate-weak close to the core. A distinct area of weak protein is evident above this region (enlarged circular inset). g Gleason 3 + 4 tumor with both PTEN gene intact in core 1 and protein levels were moderate in all areas containing tumor. h Gleason 3 + 4 tumor with both PTEN gene intact in cores 1 and 2 and protein levels were moderate in most of the areas containing tumor. However, two distinct regions arrowed and enlarged as circular insets exhibit weak intratumoral PTEN levels. The tumor region that presents PTEN loss adjacent to core 1 was classified as intraductal carcinoma. Another tumor region adjacent to core 2 shows glands with PTEN loss surrounded by PTEN intact glands (moderate and strong immunostaining) was classified as usual acinar adenocarcinoma (Gleason pattern 3). i Gleason 3 + 4 tumor with both PTEN gene intact in core 1 and protein levels were moderate in all areas containing tumor. j Gleason 3 + 4 tumor with both PTEN gene intact in core 1 and protein levels were moderate in all areas containing tumor