Fig. 2From: Use of uracil-DNA glycosylase enzyme to reduce DNA-related artifacts from formalin-fixed and paraffin-embedded tissues in diagnostic routineSequencing alignments of true and artifactual variants in the KRAS gene. Two samples (A11 and K2568), untreated and treated with UDG enzyme, were selected as representative samples of identified variants. Artifactual variant c.38G > A in melanoma sample A11 was eliminated with UDG treatment. True variant c.35G > A in colorectal cancer sample K2568 was maintained after UDG treatment. As KRAS is a gene coded in the minus strand, both variants appear as cytosine (C) to thymine (T) changes in the nucleotide sequenceBack to article page