Fig. 3

Overview of mutations in primary tumors with multiple metastases. Data of samples with sufficient coverage on nearly all genes. a: 10 patients with both liver and lung metastases; 1 discordant patient with a PIK3CA alteration limited to the primary tumor. b: 5 patients with multiple liver metastases; 1 discordant patient with two different KRAS mutations in the primary tumor, of which one mutation was also detected in both metastases. This patient also had a PIK3CA alteration limited to the primary tumor. c: 5 patients with multiple lung metastases; 2 discordant patients; 1 patient with a KRAS alteration limited to both metastases, 1 patient with a NRAS mutation limited to one of the metastasis. Grey bars: insufficient unique reads to confidently classify the multiple paired sample as wildtype or mutant (due to insufficient reads of one of the metastases)