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Fig. 6 | Applied Cancer Research

Fig. 6

From: mRNAs biomarker related to the control of proliferation and cell death in HepG2/C3A spheroid and monolayer cultures treated with piperlongumine

Fig. 6

Cytologic analysis in monolayer culture. Micrographs obtained after 24 h of treatment using the microscope EVOS FL. In the left column are the treatments containing PLN in concentrations of 0 (a), 10 (b), 20 (c) and 40 μM (d) by phase contrast microscopy. Observe the morphological changes and reduction of the cell density of the cultures that received treatments containing PLN. In the right column are the same quadrants referred to the left side seen in the fluorescence microscopy; in blue are seen the chromatin marked with Hoechst 33342, and in red the marking using rhodamine. The arrows indicate the nuclei in like-rosette format and the arrow head the nuclei with morphological patterns of apoptosis (picnosis or apoptotic bodies); observe the reduction in the intensity of the red in treatments containing PLN. e interphase cell; f apoptotic cell (picnosis); g like-rosette

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Applied Cancer Research

ISSN: 1980-5578