Fig. 9From: mRNAs biomarker related to the control of proliferation and cell death in HepG2/C3A spheroid and monolayer cultures treated with piperlongumineReactive oxygen species analysis. ROS detection after 24 h of PLN treatment. Cell were incubated with DCFDA and analyzed by flow cytometry. TB: positive control with tert-butil 200 μM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 indicate significant difference in relation to controlBack to article page