| NGS | Single test | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) | Cohen’s kappa (κ) | |
---|---|---|---|---|---|---|---|---|---|
(+) | (−) | ||||||||
EGFR mutation (n = 75) |  | Multiplex Real-Time PCR |  |  |  |  |  |  | |
*(+) | 16 | 0 | 88.89 (65.29–98.62) | 100 (93.73–100) | 100 | 96.61 (88.53–99.06) | 97.33 (90.70–99.68) | 0.92 (0.82–1.00) | |
(−) | 2 | 57 |  |  |  |  |  | Almost perfect agreement | |
ALK1 rearrangement (n = 49) |  | IHC D5F3 assay |  |  |  |  |  |  | |
(+) | 4 | 0 | 80 (28.36–99.49) | 100 (91.96–100) | 100 | 97.78 (88.4–99.61) | 97.96 (89.15–99.95) | 0.88 (0.64–1.00) | |
(−) | 1 | 44 |  |  |  |  |  | Almost perfect agreement | |
ROS1 fusion (n = 43) |  | FISH |  |  |  |  |  |  | |
(+) | 0 | 1 | – | 97.67 (87.71–99.94) | – | 100 | 97.67 (87.71–99.94) | – | |
(−) | 0 | 42 |  |  |  |  |  |  | |
c-MET amplification (n = 62) |  | FISH |  |  |  |  |  |  | |
(+) | 0 | 0 | – | 100 (93.84–100) | – | 93.55 | 93.55 (84.30–98.21) | – | |
(−) | 4 | 58 |  |  |  |  |  |  |